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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
TBC1 domain family member 13, Tbc1d13
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse TBC1 domain family member 13, TBC1D13 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
TBC1D13,TBC1 domain family member 13; TBC1 domain family, member 13
Search name
Mouse TBC1D13 ELISA KIT ,Mouse TBC1 domain family member 13 ELISA KIT ,Mouse TBC1 domain family member 13 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse TBC1 domain family member 13, TBC1D13 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to TBC1 domain family member 13. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TBC1 domain family member 13 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TBC1 domain family member 13, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TBC1 domain family member 13 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验TRAF‐Mediated TNFR‐Family Signaling
, A.M., Dowd, A.D., Brush, A.D., Heldens, A.D., Schow, A.D., Goddard, A.D., Wood, W.I., Baker, K.P., Godowski, P.J., and Ashkenazi, A. 1999. Identification of a new member of the tumor necrosis factor family and its receptor, a human ortholog of mouse
The 65-kDa oncofetal protein (p65), a novel tumor marker (1 –7 ), is highly conserved m different species (2 ,4 ). We have identified the p65 gene as a novel member of the family of genes that encode receptors for steroid hormones
, with a single mammalian family member, Argonaute2, being responsible for messenger RNA cleavage activity. This protein is essential for mouse development, and cells lacking Argonaute2 are unable to mount an experimental response to siRNAs. Mutations
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