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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Suppressor of fused homolog, Sufu
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Suppressor of fused homolog, Sufu ELISA KIT
Product Name:Mouse Suppressor of fused homolog, Sufu ELISA KIT
Packing:96T
Catalog No.:ELI-29930m
Gene Name:Mouse Sufu
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Sufu
Alternative Name:Mouse Suppressor of fused homolog, Sufu
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Suppressor of fused homolog, Sufu ELISA KIT allows for the in vitro quantitative determination of Mouse Sufu concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Suppressor of fused homolog, Sufu ELISA KIT has been pre-coated with an Mouse Suppressor of fused homolog, Sufu antibody specific to Mouse Sufu .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Sufu and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Sufu , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Sufu in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
3 suppressor of mif two 3 homolog 2 (yeast) (SMT3H2), mRNA 2940 NM_005726 Homo sapiens Ts translation elongation factor, mitochondrial (TSFM), mRNA 408 NM_005347 Homo sapiens heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) (HSPA5), mRNA 1111
Two-hybrid analysis of genetic regulatory networks
to larger sets, we and others began devising ways to do so. Larger scale two hybrid approaches typically rely on interaction mating. In this method the protein fused to the DNA-binding domain (the bait) and the protein fused to the activation domain
Studies of the Ubiquitin Proteasome System
and Purification of Anti‐Ubiquitin Antibodies Support Protocol 3: Preparation of Ubiquitin Affinity Column Generation of Reagent‐Grade Ubiquitin Activating Enzyme (E1) Basic Protocol 20: Preparation of Wheat E1 in E. coli Basic Protocol 21: Preparation of Mouse E1
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