Neuronal-specific septin-3, Se
pt3 ELISA KIT/ Mouse Neuronal-specific septin-3, Sept3 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-29777m
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Neuronal-specific septin-3, Sept3
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Neuronal-specific septin-3, SEPT3 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Neuronal-specific septin-3,SEPT3,septin 3,SEP3; bK250D10.3; neuronal-specific septin 3; neuronal-specific septin-3
Search name
Mouse Neuronal-specific septin-3 ELISA KIT ,Mouse SEPT3 ELISA KIT ,Mouse septin 3 ELISA KIT ,Mouse SEP3 ELISA KIT ,Mouse bK250D10.3 ELISA KIT ,Mouse neuronal-specific septin 3 ELISA KIT ,Mouse neuronal-specific septin-3 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse neuronal-specific septin-3,Neuronal-specific septin-3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuronal-specific septin-3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Neuronal-specific septin-3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Neuronal-specific septin-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Neuronal-specific septin-3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
Transgenic Mouse and Transgenic Rat Outline
and we will purify the DNA for microinjection from the digest. We use the Nucleospin Extract Kit for purification of microinjection DNA. Please note, if you want use large DNA fragments such as bacterial artificial chromosomes, that there is a specific protocol
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
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