Neuronal-specific septin-3, Se

Mouse Neuronal-specific septin-3, SEPT3 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Neuronal-specific septin-3,SEPT3,septin 3,SEP3; bK250D10.3; neuronal-specific septin 3; neuronal-specific septin-3

Search name

Mouse Neuronal-specific septin-3 ELISA KIT ,Mouse SEPT3 ELISA KIT ,Mouse septin 3 ELISA KIT ,Mouse SEP3 ELISA KIT ,Mouse bK250D10.3 ELISA KIT ,Mouse neuronal-specific septin 3 ELISA KIT ,Mouse neuronal-specific septin-3 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse neuronal-specific septin-3,Neuronal-specific septin-3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuronal-specific septin-3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Neuronal-specific septin-3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Neuronal-specific septin-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Neuronal-specific septin-3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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