Tyrosine-protein kinase SgK223
, Sgk223 ELISA KIT/ Mouse Tyrosine-protein kinase SgK223, Sgk223 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-29688m
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Tyrosine-protein kinase SgK223, Sgk223
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Tyrosine- protein kinase SgK223, Sgk223 ELISA KIT
Product Name:Mouse Tyrosine- protein kinase SgK223, Sgk223 ELISA KIT
Packing:96T
Catalog No.:ELI-29688m
Gene Name:Mouse Sgk223
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Sgk223
Alternative Name:Mouse Tyrosine-protein kinase SgK223, Sgk223
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Tyrosine- protein kinase SgK223, Sgk223 ELISA KIT allows for the in vitro quantitative determination of Mouse Sgk223 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Tyrosine- protein kinase SgK223, Sgk223 ELISA KIT has been pre-coated with an Mouse Tyrosine-protein kinase SgK223, Sgk223 antibody specific to Mouse Sgk223 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Sgk223 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Sgk223 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Sgk223 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验原理。PKC底物被包被在酶标板上，在ATP存在的前提下，PKC激酶将底物磷酸化，洗掉游离的PKC激酶和其他成分。再加入特异性磷酸化抗体（只识别磷酸化底物，而与非磷酸化底物无交叉反应。）检测残留在酶标板上的磷酸化底物。通过酶标二抗放大信号，读出OD值（蛋白激酶活性与OD值的对数成正比）。与对照组相比，得出处理组的蛋白激酶活性 （PKA, PKB, PKC, SGK, S6K和Tyrosine Kinase）等非放射性蛋白激酶分析测试盒http://www.immunechem.com 本文
An In Vitro Assay for the Kinase Activity of mTOR Complex 2
The mTOR complex 2 (mTORC2) is a protein kinase complex involved in many important physiological processes through the regulation of its substrates, such as Akt, SGK1, and conventional PKC. Its activity is modulated negatively by interaction
Protein Secondary Structure Prediction
Chou, P.Y. and Fasman, G.D. 1974b. Prediction of protein conformation. Biochemistry 13:223‐245. Chou, P.Y. and Fasman, G.D. 1977. β‐turns in proteins. J. Mol. Biol. 115:135
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