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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Transmembrane protein 182, Tmem182
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Transmembrane protein 182, Tmem182 ELISA KIT
Product Name:Mouse Transmembrane protein 182, Tmem182 ELISA KIT
Packing:96T
Catalog No.:ELI-29388m
Gene Name:Mouse Tmem182
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Tmem182
Alternative Name:Mouse Transmembrane protein 182, Tmem182
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Transmembrane protein 182, Tmem182 ELISA KIT allows for the in vitro quantitative determination of Mouse Tmem182 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Transmembrane protein 182, Tmem182 ELISA KIT has been pre-coated with an Mouse Transmembrane protein 182, Tmem182 antibody specific to Mouse Tmem182 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Tmem182 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Tmem182 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Tmem182 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Semiquantitative immunoblots of membrane protein-epidermal growth factor
Membrane proteins or cytokines are sometimes difficult to isolate and purify. Our group recently concentrated on epidermal growth factor (EGF) protein expression studies. Mature EGF was initially identified from mouse submaxillary gland extract
Synchronizing Protein Transport in the Secretory Pathway
, Interleukin‐2 signal sequence; SBP, streptavidin‐binding peptide; X, protein of interest. View Image
Membrane‐Based Yeast Two‐Hybrid System to Detect Protein Interactions
proteins are anchored to the membrane by an N‐terminal fusion to the small transmembrane protein Ost4p. The Cub‐LexA‐VP16 cassette is fused to the C‐terminus of the soluble protein of interest. (C ) An interacting protein (the prey) is expressed as a fusion
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