Probable E3 ubiquitin-protein

Mouse Probable E3 ubiquitin- protein ligase MID2, Mid2 ELISA KIT

Product Name:Mouse Probable E3 ubiquitin- protein ligase MID2, Mid2 ELISA KIT
Packing:96T

Catalog No.:ELI-28926m

Gene Name:Mid2

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:Mouse Probable E3 ubiquitin- protein ligase MID2

Alternative Name:Mid2,Mouse Probable E3 ubiquitin- protein ligase MID2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Probable E3 ubiquitin- protein ligase MID2, Mid2 ELISA KIT allows for the in vitro quantitative determination ofMouse Probable E3 ubiquitin- protein ligase MID2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Probable E3 ubiquitin- protein ligase MID2, Mid2 ELISA KIT has been pre-coated with an Mid2 antibody specific to Mouse Probable E3 ubiquitin- protein ligase MID2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Probable E3 ubiquitin- protein ligase MID2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Probable E3 ubiquitin- protein ligase MID2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Probable E3 ubiquitin- protein ligase MID2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

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