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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Testis-expressed protein 19.2, Tex19.2
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Testis- expressed protein 19.2, Tex19.2 ELISA KIT
Product Name:Mouse Testis- expressed protein 19.2, Tex19.2 ELISA KIT
Packing:96T
Catalog No.:ELI-28286m
Gene Name:Mouse Tex19.2
Detect Range:15.62-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Tex19.2
Alternative Name:Mouse Testis-expressed protein 19.2, Tex19.2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Testis- expressed protein 19.2, Tex19.2 ELISA KIT allows for the in vitro quantitative determination of Mouse Tex19.2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Testis- expressed protein 19.2, Tex19.2 ELISA KIT has been pre-coated with an Mouse Testis-expressed protein 19.2, Tex19.2 antibody specific to Mouse Tex19.2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Tex19.2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Tex19.2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Tex19.2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Transgenic Mouse and Transgenic Rat Outline
to show that transgene is expressed. RNA expression can be detected by in situ hybridization or RNAse protection assay with RNA probes. Alternatively, an RT-PCR approach can be used. The protein that is produced must be different from proteins normally expressed
Estrogen-Deficient Mouse Models in the Study of Brain Injury and Disease
into the mitochondrion of steroidogenic cells (1-3). This transfer of cholesterol into the mitochondrion is facilitated by the steroidogenic acute regulatory protein (StAR) (1). This is the rate-limiting step in androgen biosynthesis, as the cholesterol
包涵体表达蛋白的纯化方法Purification of Expressed Proteins from Inclusion Bodies
ABSTRACT The expression of foreign proteins at high levels in E. coli often results in the formation of inclusion bodies composed of insoluble aggregates of the expressed protein. The inclusion bodies
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