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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Tsukushin, Tsku
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Tsukushin, TSKU ELISA KIT
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
TSKU,tsukushi,Tsukushin, small leucine rich proteoglycan; tsukushi small leucine rich proteoglycan homolog; tsukushi homolog; E2IG4; LRRC54; TSK; E2-induced gene 4 protein; leucine rich repeat containing 54; leucine-rich repeat-containing protein 54,
Search name
Mouse TSKU ELISA KIT ,Mouse tsukushi ELISA KIT ,Mouse Tsukushin ELISA KIT ,Mouse small leucine rich proteoglycan ELISA KIT ,Mouse tsukushi small leucine rich proteoglycan homolog ELISA KIT ,Mouse tsukushi homolog ELISA KIT ,Mouse E2IG4 ELISA KIT ,Mouse LRRC54 ELISA KIT ,Mouse TSK ELISA KIT ,Mouse E2-induced gene 4 protein ELISA KIT ,Mouse leucine rich repeat containing 54 ELISA KIT ,Mouse leucine-rich repeat-containing protein 54 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Tsukushin concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
The ELISA is based on the competitive binding enzyme immunoassaytechnique. The microtiter plate provided in this kit has been pre-coated with an antibody specific toTsukushi, During the reaction,Tsukushiin the sample or standard competes with a fixed amount of biotin-labeledTsukushi for sites on a pre-coated Monoclonal antibody specific to Tsukushi. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Tsukushi in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
Performing Bronchoalveolar Lavage in the Mouse
and inflammatory cell populations; it is indispensable for studying cell influx in disease models of the airways such as asthma and COPD. Cell counts can be combined with methods such as ELISA, immunoblot, immunohistochemistry, quantitative polymerase chain
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
from invertebrates to lower and higher verterbrates, and intervenes both in physiological and in pathological states, such as normal cell turnover, embryogenesis, metamorphosis, thymic maturation and involution, neoplasm, etc. (1-4). A mouse anti-DNA monoclonal
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