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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Telomeric repeat-binding factor 2, Terf2
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Telomeric repeat- binding factor 2, Terf2 ELISA KIT
Product Name:Mouse Telomeric repeat- binding factor 2, Terf2 ELISA KIT
Packing:96T
Catalog No.:ELI-29033m
Gene Name:Mouse Terf2
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Terf2
Alternative Name:Mouse Telomeric repeat-binding factor 2, Terf2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Telomeric repeat- binding factor 2, Terf2 ELISA KIT allows for the in vitro quantitative determination of Mouse Terf2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Telomeric repeat- binding factor 2, Terf2 ELISA KIT has been pre-coated with an Mouse Telomeric repeat-binding factor 2, Terf2 antibody specific to Mouse Terf2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Terf2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Terf2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Terf2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Numerous studies on telomerase expression have consistently demonstrated the presence of telomerase activity in the vast majority of different types of cancer, as well as immortalized cells, but have failed to detect telomerase in most normal
The Monitoring and Affinity Purification of Proteins Using Dual Tags with Tetracysteine Motifs
via a system built with tetracysteine motifs and various combinations of affinity tags. Using human telomeric repeat binding factor 2 (TRF2) as an example, we demonstrate the power of the system in terms of bait protein recovery after dual-tag affinity purification
restriction site polymorphisms and/or subtelomeric length polymorphisms that may confound the identification of primary factors accounting for inter-individual variation in the mean length of the true telomeric repeat sequence. More recently, methods
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