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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Leukotriene C4 synthase, Ltc4s
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Leukotriene C4 synthase, LTC4S ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Leukotriene C4 synthase,LTC4 synthase,Leukotriene-C(4) synthase,LTC4s,leukotriene C4 synthase,LTC4 synthase
Search name
Mouse Leukotriene C4 synthase ELISA KIT,Mouse LTC4 synthase ELISA KIT,Mouse Leukotriene-C(4) synthase ELISA KIT ,Mouse LTC4s ELISA KIT,Mouse leukotriene C4 synthase ELISA KIT ,Mouse LTC4 synthase ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse leukotriene C4 synthase,LTC4 synthase concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to LTC4 synthase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for LTC4 synthase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain LTC4 synthase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of LTC4 synthase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Extraction and Measurements of Prostanoids and Leukotrienes by Radioimmunoassays
in the lipoxygenase (LOX) and cyclo-oxygenase (COX or PGHS) pathways, such as the leukotriene C4 synthase and the novel inducible COX enzyme named COX-2, and the cloning of receptors to eicosanoids. The discovery of the inducible prostaglandin synthase, COX-2 or PGHS
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