- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-27487m
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Granzyme D, Gzmd
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Granzyme D ELISA KIT, Gzmd ELISA Kit (CUSTOM)
96 Tests
Operating instructions
FOR RESEARCH USE ONLY!
Alternative name
Mouse Gzmd ELISA KIT ,Mouse CCP5 ELISA KIT ,Mouse Ctla-5 ELISA KIT ,Mouse Ctla5 ELISA KIT ,Mouse granzyme D ELISA KIT
Intended use
Mouse Gzmd ELISA kit allows for the in vitro quantitative determination of Mouse Granzyme D concentrations in serum ELISA KIT, plasma ELISA KIT, tissue homogenates ELISA KIT, cell culture supernates ELISA KIT, and other biological fluids.
Test principle
Mouse Gzmd ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gzmd ELISA KIT, During the reaction ELISA KIT, Gzmd in the sample or standard competes with a fixed amount of biotin-labeled Gzmd for sites on a pre-coated Monoclonal antibody specific to Gzmd. Excess conjugate and unbound sample or standard are washed from the plate. Next ELISA KIT, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Gzmd in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product Min.Order 5kits
Delivery time 4-6month
Order Mouse Gzmd ELISA kit by email:
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文献和实验Human/Mouse/rat Neural lineage Functional identification Kit（目录号 Sc028） 在神经祖细胞（NPc）的分离和扩增过程中，必须通过评估它们分化成多个神经谱系的能力，从而在功能上评估它们的多能性。本文介绍的试剂盒提供了一种快速简单的技术，可确认您的起始细胞群向星形胶质细胞、神经元和少突胶质细胞谱系分化的能力。 在实验过程的早期阶段验证多能性的能力： √ 定义起始干细胞群体 √ 减少实验中以及不同研究之间的可
, dry 5. block half of the plate (with platelets) by PBS-BSA 1% and coat another half by glycine (x1) - BSA for 1 hour at 37ѓC 6. wash 3 times in PBS-tween 20, dry 7. incubated at 37ѓc for 1 hour with a 1:1000 dilution of rat IgG2a anti-mouse kappa
A. Formalin Fixed Cell Plates 1. Trypsinize confluent flasks 2. Pool and count cells 3. Centrifuge at 1500 rpm for 10 minutes 4. Resuspend to the appropriate concentration in complete medium4 x 105 cells/ml for epithelial cells
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