Golgi reassembly-stacking prot
ein 1, Gorasp1 ELISA KIT/ Mouse Golgi reassembly-stacking protein 1, Gorasp1 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-27464m
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Golgi reassembly-stacking protein 1, Gorasp1
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Golgi reassembly- stacking protein 1, Gorasp1 ELISA KIT
Product Name:Mouse Golgi reassembly- stacking protein 1, Gorasp1 ELISA KIT
Packing:96T
Catalog No.:ELI-27464m
Gene Name:Mouse Gorasp1
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Gorasp1
Alternative Name:Mouse Golgi reassembly-stacking protein 1, Gorasp1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Golgi reassembly- stacking protein 1, Gorasp1 ELISA KIT allows for the in vitro quantitative determination of Mouse Gorasp1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Golgi reassembly- stacking protein 1, Gorasp1 ELISA KIT has been pre-coated with an Mouse Golgi reassembly-stacking protein 1, Gorasp1 antibody specific to Mouse Gorasp1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Gorasp1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Gorasp1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Gorasp1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Immunohistochemisty-Fluorescence Protocol
morphology of the juxtanuclear staining pattern generated by the accumulation of the cytokines in the Golgi organelle. Sample Preparation and Fixation Harvest cells and wash twice in V-bottomed tubes with cold Wash Buffer by centrifugation (400 x
Immunohistochemistry: Fluorescence Protocol 2.0
by centrifugation (400 x g for 5 minutes). Incubate the cells for 30 minutes at room temperature with 50 µL of a biotinylated secondary antibody (either biotin-donkey anti-goat IgG Fab2 diluted 1:700 or biotin-goat anti-mouse IgG1 or IgG2A or IgG2B diluted
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of interest as the solvents can partially dissolve indigo. For glutaraldehyde-fixed mouse retina, which is approximately 250 microns thick, the following procedure was used. Dehydrate through graded ethanols (50%, 70%, 95%, 100%, 100%) for 20 min each. Clear in xylene, 2 x 15
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