- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-26621m
- 2025年07月07日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Frizzled-4, Fzd4
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Frizzled- 4, Fzd4 ELISA KIT
Product Name:Mouse Frizzled- 4, Fzd4 ELISA KIT
Packing:96T
Catalog No.:ELI-26621m
Gene Name:Mouse Fzd4
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Fzd4
Alternative Name:Mouse Frizzled-4, Fzd4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Frizzled- 4, Fzd4 ELISA KIT allows for the in vitro quantitative determination of Mouse Fzd4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Frizzled- 4, Fzd4 ELISA KIT has been pre-coated with an Mouse Frizzled-4, Fzd4 antibody specific to Mouse Fzd4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Fzd4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Fzd4 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Fzd4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验。Triton-X-100抽提Frizzled-4效果很差，相反，TM-PEK试剂A得到的Frizzled-4在western blot中获得了明显的信号。而TM-PEK试剂对CELSR-3的抽提效果非常令人惊喜，这种全长的358kD蛋白只有TM-PEK试剂A才能获得，而SDS或Triton X-100效果都使人失望。 2． Thermo旗下Pierce的膜蛋白分离试剂盒（货号89881）——该试剂盒首先采用biotin标记膜蛋白（该膜蛋白一般需要有暴露在膜外的赖氨酸残基和足够长度的膜外
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
Performing Bronchoalveolar Lavage in the Mouse
and inflammatory cell populations; it is indispensable for studying cell influx in disease models of the airways such as asthma and COPD. Cell counts can be combined with methods such as ELISA, immunoblot, immunohistochemistry, quantitative polymerase chain
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