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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Probable RNA-binding protein EIF1AD, Eif1ad
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Probable RNA- binding protein EIF1AD, Eif1ad ELISA KIT
Product Name:Mouse Probable RNA- binding protein EIF1AD, Eif1ad ELISA KIT
Packing:96T
Catalog No.:ELI-26582m
Gene Name:Mouse Eif1ad
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Eif1ad
Alternative Name:Mouse Probable RNA-binding protein EIF1AD, Eif1ad
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Probable RNA- binding protein EIF1AD, Eif1ad ELISA KIT allows for the in vitro quantitative determination of Mouse Eif1ad concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Probable RNA- binding protein EIF1AD, Eif1ad ELISA KIT has been pre-coated with an Mouse Probable RNA-binding protein EIF1AD, Eif1ad antibody specific to Mouse Eif1ad .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Eif1ad and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Eif1ad , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Eif1ad in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Reproducible purification of DNA and RNA in 96-well format. Genomic DNA and total RNA were purified from mouse tissues using the AllPrep DNA/RNA 96 Kit. A Purified DNA samples were analyzed on a 0.8% agarose gel. B
Isolation of RNA from Difficult Tissues
ToTALLY RNA™ Kit). Note that many of these techniques can be used with other RNA isolation protocols as well. Heart and Skeletal Muscle: Fibrous Tissues For fibrous tissues such as rat and mouse heart and skeletal muscle, the most difficult step
Potyvirus Isolation and RNA Extraction
of CP of ca. 30–36 kDa. This RNA (ca. 8500–9800 nucleotides) is polyadenylated and also contains a VPg covalently bound to the 5′ end. Coat protein amino acid sequences show significant homology, particularly within the core region. Virions are approx
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