Mouse Desmoplakin, DSP ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
DSP,DPI; DPII; 250/210 kDa paraneoplastic pemphigus antigen; desmoplakin I; desmoplakin II,Desmoplakin,desmoplakin II; desmoplakin I; DPI; DPII; 250/210 kDa paraneoplastic pemphigus antigen
Search name
Mouse DSP ELISA KIT ,Mouse DPI ELISA KIT ,Mouse DPII ELISA KIT ,Mouse 250/210 kDa paraneoplastic pemphigus antigen ELISA KIT ,Mouse desmoplakin I ELISA KIT ,Mouse desmoplakin II ELISA KIT ,Mouse Desmoplakin ELISA KIT ,Mouse desmoplakin II ELISA KIT ,Mouse desmoplakin I ELISA KIT ,Mouse DPI ELISA KIT ,Mouse DPII ELISA KIT ,Mouse 250/210 kDa paraneoplastic pemphigus antigen ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Desmoplakin, DSP beta concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Desmoplakin, DSP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Mouse Desmoplakin, DSP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Desmoplakin, DSP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse Desmoplakin, DSP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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