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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
DENN domain-containing protein 1C, Dennd1c
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse DENN domain- containing protein 1C, Dennd1c ELISA KIT
Product Name:Mouse DENN domain- containing protein 1C, Dennd1c ELISA KIT
Packing:96T
Catalog No.:ELI-26367m
Gene Name:Mouse Dennd1c
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Dennd1c
Alternative Name:Mouse DENN domain-containing protein 1C, Dennd1c
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse DENN domain- containing protein 1C, Dennd1c ELISA KIT allows for the in vitro quantitative determination of Mouse Dennd1c concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse DENN domain- containing protein 1C, Dennd1c ELISA KIT has been pre-coated with an Mouse DENN domain-containing protein 1C, Dennd1c antibody specific to Mouse Dennd1c .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Dennd1c and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Dennd1c , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Dennd1c in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Prediction of Protein‐Protein Interaction Networks
., Sabatti, C., and Eisenberg, D. 2005. Inferring protein domain interactions from databases of interacting proteins. Genome Biol. 6:R89. Sato, T., Yamanishi, Y., Kanehisa, M
Mapping Protein‐Protein Interactions with Phage‐Displayed Combinatorial Peptide Libraries
Figure 17.4.1 Photograph of a phage ELISA result. Using eight different clones that were isolated with GST‐SrcSH3 domain as the target, 25 µl of culture supernatant were added to pairs of microtiter plate wells containing GST‐SrcSH3 or GST
purification of protein complexes, in combination with in vivo biotinylation of critical transcription factors, has contributed to the analysis of the pluripotent state in mouse embryonic stem (ES) cells and made it possible to construct a protein?protein
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