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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Collagen alpha-1(XXIII) chain, Col23a1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Collagen alpha- 1(XXIII) chain, COL23A1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
COL23A1,collagen alpha-1(XXIII) chain; procollagen, type XXIII, alpha 1; collagen, type XXIII, alpha 1
Search name
Mouse COL23A1collagen alpha-1(XXIII) chain ELISA KIT ,Mouse procollagen type XXIII alpha 1 ELISA KIT ,Mouse collagen type XXIII alpha 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Collagen alpha- 1(XXIII) chain, COL23A1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Collagen alpha- 1(XXIII) chain,COL23A1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Mouse Collagen alpha- 1(XXIII) chain,COL23A1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Collagen alpha- 1(XXIII) chain,COL23A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse Collagen alpha- 1(XXIII) chain,COL23A1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product
Delivery time 4-6month
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文献和实验The enzyme-linked immunosorbent assay (ELISA) is a widely used technique for detecting antibodies (Abs) and is employed in clinical laboratories to identify Abs against various self-antigens–autoAb development and quantitation. This method
CD49a/CD49b/CD49c/CD49d/CD49e/CD49f分子
and collagen receptor Positive staining (normal): activated B and T cells, monocytes CD49b Aka very late antigen (VLA) alpha 2 chain - on T cells Aka GPIa/IIa when expressed on platelets Receptor for laminin, collagen
Performing Bronchoalveolar Lavage in the Mouse
and inflammatory cell populations; it is indispensable for studying cell influx in disease models of the airways such as asthma and COPD. Cell counts can be combined with methods such as ELISA, immunoblot, immunohistochemistry, quantitative polymerase chain
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