- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-25966m
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
D7h11orf16
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse D7h11orf16 ELISA KIT
Product Name:Mouse D7h11orf16 ELISA KIT
Packing:96T
Catalog No.:ELI-25966m
Gene Name:Mouse D7h11orf16
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse D7h11orf16
Alternative Name:Mouse D7h11orf16
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse D7h11orf16 ELISA KIT allows for the in vitro quantitative determination of Mouse D7h11orf16 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse D7h11orf16 ELISA KIT has been pre-coated with an Mouse D7h11orf16 antibody specific to Mouse D7h11orf16 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse D7h11orf16 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse D7h11orf16 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse D7h11orf16 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
、洗液不够时，可用蒸馏水自行配制PH7.4，0.02M的磷酸缓冲液，加入0.1%的吐温20作为洗液。加入1/1000的叠氮钠后可长期保存。12、底物是光敏感的，要在临用前现配。13、检测前，要打开酶标仪，使之稳定10分钟以上。14、底物有一定的毒性，终止液对皮肤有腐蚀性，应尽量避免接触。15、待检样品要澄清，否则会影响结果。16、温浴时间应遵守试剂盒规定。17、应尽量做双孔实验，这样才能保证数据的准确性。18、对结果有疑问的样品要用其它方法进行确证。二、在使用ELISA试剂盒时，出现问题怎么办？下面
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