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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Thiomorpholine-carboxylate dehydrogenase, Crym
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Thiomorpholine- carboxylate dehydrogenase, CRYM ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
CRYM,DFNA40; THBP; NADP-regulated thyroid-hormone binding protein; ketimine reductase; mu-crystallin homolog; thiomorpholine-carboxylate dehydrogenase; crystallin, mu
Search name
Mouse CRYM ELISA KIT ,Mouse DFNA40 ELISA KIT ,Mouse THBP ELISA KIT ,Mouse NADP-regulated thyroid-hormone binding protein ELISA KIT ,Mouse ketimine reductase ELISA KIT ,Mouse mu-crystallin homolog ELISA KIT ,Mouse thiomorpholine-carboxylate dehydrogenase ELISA KIT ,Mouse crystallin mu ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Thiomorpholine- carboxylate dehydrogenase, CRYM concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to CRYM. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CRYM and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CRYM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CRYM in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
Transplantation Models to Characterize the Mechanisms of Stem Cell–Induced Islet Regeneration
our current knowledge regarding the isolation human bone marrow?derived progenitor cells for the paracrine stimulation of islet regeneration after transplantation into immunodeficient mouse models of diabetes. By using high aldehyde dehydrogenase (ALDHhi
a subset of genomic DNA sequences of type 5'-(NNN )3 -3', whereas the 6ZF library would recognize a subset of genomic sequences of type 5'-(NNN )6 -3'. Given the zinc-finger domains used, both libraries were more likely to recognize (RNN)x -type sequences
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