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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
UPF0684 protein C5orf30 homolog, D1Ertd622e
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse UPF0684 protein C5orf30 homolog, D1Ertd622e ELISA KIT
Product Name:Mouse UPF0684 protein C5orf30 homolog, D1Ertd622e ELISA KIT
Packing:96T
Catalog No.:ELI-25701m
Gene Name:Mouse D1Ertd622e
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse D1Ertd622e
Alternative Name:Mouse UPF0684 protein C5orf30 homolog, D1Ertd622e
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse UPF0684 protein C5orf30 homolog, D1Ertd622e ELISA KIT allows for the in vitro quantitative determination of Mouse D1Ertd622e concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse UPF0684 protein C5orf30 homolog, D1Ertd622e ELISA KIT has been pre-coated with an Mouse UPF0684 protein C5orf30 homolog, D1Ertd622e antibody specific to Mouse D1Ertd622e .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse D1Ertd622e and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse D1Ertd622e , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse D1Ertd622e in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Studies of the Ubiquitin Proteasome System
Basic Protocol 9: Substrate Ubiquitylation by Multisubunit E3s Materials VBC‐Cul2 E3 protein complex expressed and purified using baculoviral expression system ( protocol 45 ) Mouse E1
of protein-DNA contacts. (B) Screening for functional TFZF activators in A431 cells. (C�F) Flow cytometric analysis of A431 cells infected with some of the selected pMX-TFZF pools from the 3ZF selections (C, D) or 6ZF selections (E, F). Shown are upregulation
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