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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Cysteine-rich secretory protein 1, Crisp1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Cysteine- rich secretory protein 1, Crisp1 ELISA KIT
Product Name:Mouse Cysteine- rich secretory protein 1, Crisp1 ELISA KIT
Packing:96T
Catalog No.:ELI-25698m
Gene Name:Mouse Crisp1
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Crisp1
Alternative Name:Mouse Cysteine-rich secretory protein 1, Crisp1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Cysteine- rich secretory protein 1, Crisp1 ELISA KIT allows for the in vitro quantitative determination of Mouse Crisp1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Cysteine- rich secretory protein 1, Crisp1 ELISA KIT has been pre-coated with an Mouse Cysteine-rich secretory protein 1, Crisp1 antibody specific to Mouse Crisp1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Crisp1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Crisp1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Crisp1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Isolation of RNA from Difficult Tissues
. Therefore, care should be taken during the initial preparation of the homogenate to ensure intact total RNA. Brain and Plant Tissues: Protein and Lipid-rich Tissues Isolating total RNA from rat or mouse brain can be very rewarding
Models and Analysis of Atherosclerosis, Restenosis, and Aneurysm Formation in the Mouse
Normal mouse serum (NMS, Sigma) AEC kit (Vector Laboratories) Hematoxylin (Sigma
Antigen Design Sera Purification Tech Sheet
to the carrier protein. For synthesis purposes we recommend that the placement of cysteine be at the N-terminus of the peptide if possible. Glutaraldehyde is a bifunctional coupling reagent that links two compounds through their amino groups. Glutaraldehyde
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