- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-25632m
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Beta-chimaerin, Chn2
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Beta- chimaerin, Chn2 ELISA KIT
Product Name:Mouse Beta- chimaerin, Chn2 ELISA KIT
Packing:96T
Catalog No.:ELI-25632m
Gene Name:Mouse Chn2
Detect Range:15.6-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Chn2
Alternative Name:Mouse Beta-chimaerin, Chn2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Beta- chimaerin, Chn2 ELISA KIT allows for the in vitro quantitative determination of Mouse Chn2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Beta- chimaerin, Chn2 ELISA KIT has been pre-coated with an Mouse Beta-chimaerin, Chn2 antibody specific to Mouse Chn2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Chn2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Chn2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Chn2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验FA Extraction of [insoluble] Amyloid beta from Mouse Brain
each sample individually for one minute on ice. Immerse the tip of the probe in the sample, then move tube up and down while sonicating. 3. Spin 400 μl at 135,000 x g for 1 hr at 4°C (50,000 rpm in a TLA 100.3 rotor). 4. Dilute 210 μl
【求助】关于TGF-Beta，FN酶联免疫（ELISA）试剂盒的选择参与者：txl4fox我是要酶联免疫（ELISA）的方法测细胞上清液中的TGF-Beta，FN抗原的表达情况，关于ELISA试剂盒的选择我想请教几个问题：我想买酶标板自己包被一抗，那我应该如何选择ELISA试剂盒？有没有不带酶标板只带一抗，二抗，酶底物，以及底物显色剂而且够做五六张板的ELISA试剂盒？如果没有，是不是我应该分开订购？ELISA试剂盒从国外哪几个公司订购比较好同时又相对不会很贵？谢谢。参与者：风来了你说的市场
，从而引起假阴性结果。此外，冻融标本的混匀亦应注意，不要进行剧烈振荡，反复颠倒混匀即可。 （5）标本在保存中如出现细菌污染所致的混浊或絮状物时，应离心沉淀后取上清检测。 二、试剂准备 在ELISA测定中试剂的准备最为关键的是，在实验开始前，将试剂盒先从冰箱中拿出来，在室温下放置20分钟以上后，再进行测定，以使试剂盒在使用前与室温平衡。这样做的目的，主要是为了在后面的温育反应步骤中，能使反应微孔内的温度能较快
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