B-cell lymphoma/leukemia 10, B
cl10 ELISA KIT/ Mouse B-cell lymphoma/leukemia 10, Bcl10 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-25204m
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
B-cell lymphoma
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse B- cell lymphoma/leukemia 10, Bcl10 ELISA KIT
Product Name:Mouse B- cell lymphoma/leukemia 10, Bcl10 ELISA KIT
Packing:96T
Catalog No.:ELI-25204m
Gene Name:Mouse Bcl10
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Bcl10
Alternative Name:Mouse B-cell lymphoma/leukemia 10, Bcl10
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse B- cell lymphoma/leukemia 10, Bcl10 ELISA KIT allows for the in vitro quantitative determination of Mouse Bcl10 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse B- cell lymphoma/leukemia 10, Bcl10 ELISA KIT has been pre-coated with an Mouse B-cell lymphoma/leukemia 10, Bcl10 antibody specific to Mouse Bcl10 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Bcl10 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Bcl10 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Bcl10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Mouse B cell isolation with magnetic beads
实验概要 Isolate B cells from total mouse cells Praparation: 1.Enough MACS; put RPMI into water bath. 2.Put some MACS in 15 ml tube or 1.5 ml tube from cell room
DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
of the reagent per 2 x 106 of cultured cells. 2) Cells Grown in Monolayer Lyse cells directly in a culture dish by adding 300 μL of Cell Lysis Buffer per 2 x 106 of cultured cells directly into each well of multiwell cell culture plate or flask
microscope. This will allow observation of the cells without opening or disturbing the growth. 16. Make cell density determinations at 10 X magnification using a square ocular grid, as explained in Chapter One for the determination of area. 17. Plot
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