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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
ATP-binding cassette sub-family B member 10, mitochondri
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse ATP- binding cassette sub- family B member 10, mitochondrial, ABCB10 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
ABCB10,EST20237; M-ABC2; MTABC2; ABC transporter 10 protein; ATP-binding cassette sub-family B member 10 mitochondrial; ATP-binding cassette transporter 10; mitochondrial ATP-binding cassette 2; ATP-binding cassette sub-family B (MDR/TAP) member 10,
Search name
Mouse ABCB10 ELISA KIT ,Mouse EST20237 ELISA KIT ,Mouse M-ABC2 ELISA KIT ,Mouse MTABC2 ELISA KIT ,Mouse ABC transporter 10 protein ELISA KIT ,Mouse ATP-binding cassette sub-family B member 10 mitochondrial ELISA KIT ,Mouse ATP-binding cassette transporter 10 ELISA KIT ,Mouse mitochondrial ATP-binding cassette 2 ELISA KIT ,Mouse ATP-binding cassette sub-family B (MDR/TAP) member 10 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse ATP- binding cassette sub- family B member 10, mitochondrial, ABCB10 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to ABCB10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ABCB10 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ABCB10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ABCB10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized Product
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文献和实验Crystallization of Kinesin Family Motor Proteins
) PEG 4000, 2.5 mM ATP, 10 mM MgCl2Reservoir: 100 mM Na-acetate pH 4.6, 150 mM KCl, 7% PEG 4000, 5 mM ATP, 20 mM MgCl2Kull et al., 1996Ncd 335-700Sitting drop Room temperature7 mg/ml protein in 10 mM Pipes, pH 7.2, 100 mM NaCl, 1 mM EGTA, 1 mM DTT, 7% (w
Characterization of Calcium Channel Binding
Figure 1.25.1 (A ) Saturation binding of [3 H]PN200‐100 to L‐type calcium channels in mouse heart membrane preparation ( n = 2). (B ) Scatchard analysis of the specific binding data: K d = 54.9 pM and B max = 116.4 fmol/mg protein
Benzodiazepine Binding to GABAA Receptors
of [3 H]flumazenil in the presence and absence of 10 µM clonazepam to determine total and nonspecific binding. (B ) Scatchard plot of specific [3 H]flumazenil binding shown in panel A. The dissociation constant ( K D ) and maximum number of binding sites
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