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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Beta-1 adrenergic receptor, Adrb1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Beta- 1 adrenergic receptor, ADRB1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Beta-1 adrenergic receptor,Beta-1 adrenoreceptor(Beta-1 adrenoceptor, ADRB1,ADRB1R; B1AR; BETA1AR; RHR; adrenergic, beta-1-, receptor; beta-1 adrenergic receptor; beta-1 adrenoceptor; beta-1 adrenoreceptor; adrenoceptor beta 1
Search name
MouseBeta-1 adrenergic receptor ELISA KIT ,MouseBeta-1 adrenoreceptor(Beta-1 adrenoceptor ELISA KIT ,Mouse ADRB1 ELISA KIT ,MouseADRB1R ELISA KIT ,MouseB1AR ELISA KIT ,MouseBETA1AR ELISA KIT ,MouseRHR ELISA KIT ,Mouseadrenergic ELISA KIT ,Mouse beta-1- ELISA KIT ,Mouse receptor ELISA KIT ,Mousebeta-1 adrenergic receptor ELISA KIT ,Mousebeta-1 adrenoceptor ELISA KIT ,Mousebeta-1 adrenoreceptor ELISA KIT ,Mouseadrenoceptor beta 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Beta-1 adrenergic receptor concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-1 adrenergic receptor. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Beta-1 adrenergic receptor and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Beta-1 adrenergic receptor, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Beta-1 adrenergic receptor in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
beta-actin B-actin GAPDH(Human, rat and mouse) GAPDH(人/小鼠/大鼠 通用) GAPDH GAPDH/ NM_002046 GAPDH(大鼠、小鼠、人) β-actin(大鼠、小鼠、人) beta-actin beta-actin PCR or RT-PCR EBV BamHⅠ-W EBV Qp EBV Wp EBV Cp EBV Fp EBV LMP1 EBV LMP2A EBV
Flow Cytometry Assay to Measure Internalization of GPCRs
engineered onto the N-terminus of the GPCR.The following protocol is optimized for internalization of FLAG-tagged beta 2-adrenergic receptor (B2AR) stably transfected into HEK293 cells.Protocol 1) Grow up receptors in 37degC incubator until confluent
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