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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Ankrd1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Ankrd1 ELISA KIT
Product Name:Mouse Ankrd1 ELISA KIT
Packing:96T
Catalog No.:ELI-24206m
Gene Name:Mouse Ankrd1
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Ankrd1
Alternative Name:Mouse Ankrd1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Ankrd1 ELISA KIT allows for the in vitro quantitative determination of Mouse Ankrd1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Ankrd1 ELISA KIT has been pre-coated with an Mouse Ankrd1 antibody specific to Mouse Ankrd1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ankrd1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ankrd1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ankrd1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验人心肌锚蛋白重复域1(ANKRD1)酶联免疫分析(ELISA)
人心肌锚蛋白重复域1 (ANKRD1 ) 酶联免疫分析(ELISA ) 试剂盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相关液体样本中心肌锚蛋白重复域1 (ANKRD1 )的 含量。 实验原理: 本试剂盒应用双抗体夹心法测定标本中人 心肌锚蛋白重复域 1 ( ANKRD1 ) 水平。用纯化的人 心肌锚蛋白重复域 1 ( ANKRD1 ) 抗体包被微孔板,制成固相抗体,往包
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
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