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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Testis-expressed protein 14, Tex14
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Testis- expressed protein 14, Tex14 ELISA KIT
Product Name:Mouse Testis- expressed protein 14, Tex14 ELISA KIT
Packing:96T
Catalog No.:ELI-23259m
Gene Name:Mouse Tex14
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Tex14
Alternative Name:Mouse Testis-expressed protein 14, Tex14
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Testis- expressed protein 14, Tex14 ELISA KIT allows for the in vitro quantitative determination of Mouse Tex14 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Testis- expressed protein 14, Tex14 ELISA KIT has been pre-coated with an Mouse Testis-expressed protein 14, Tex14 antibody specific to Mouse Tex14 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Tex14 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Tex14 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Tex14 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Transgenic Mouse and Transgenic Rat Outline
to show that transgene is expressed. RNA expression can be detected by in situ hybridization or RNAse protection assay with RNA probes. Alternatively, an RT-PCR approach can be used. The protein that is produced must be different from proteins normally expressed
Estrogen-Deficient Mouse Models in the Study of Brain Injury and Disease
tissue (7), muscle (7), bone (8), adrenal (9), vasculature, and numerous sites in the brain (10, 11). Other estrogen-synthesizing tissues include testis (12), placenta (13), fetal (but not adult) liver (14). Adipose tissue synthesizes estrone
Analysis of Phospholipid Hydroperoxide Glutathione Peroxidase mRNA
as a soluble peroxidase in spermatids, but loses its activity in mature spermatozoa and persists as an oxidatively cross-linked insoluble protein (13 ). Besides, GPX4 is expressed in all major tissues studied so far. Comparatively, GPX4 mRNA and activity
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