Mouse ADI1 (1,2- dihydroxy- 3- keto- 5- methylthiopentene dioxygenase) ELISA Kit (CUSTOM)
96 Tests
Operating instructions
FOR RESEARCH USE ONLY!
Alternative name
Mouse ADI1 ELISA KIT ,Mouse APL1 ELISA KIT ,Mouse ARD ELISA KIT ,Mouse Fe-ARD ELISA KIT ,Mouse HMFT1638 ELISA KIT ,Mouse MTCBP1 ELISA KIT ,Mouse Ni-ARD ELISA KIT ,Mouse SIPL ELISA KIT ,Mouse mtnD ELISA KIT ,Mouse MT1-MMP cytoplasmic tail-binding protein-1 ELISA KIT ,Mouse acireductone dioxygenase (Fe(2+)-requiring) ELISA KIT ,Mouse acireductone dioxygenase (Ni(2+)-requiring) ELISA KIT ,Mouse membrane-type 1 matrix metalloproteinase cytoplasmic tail binding protein-1 ELISA KIT ,Mouse submergence induced protein 2 ELISA KIT ,Mouse submergence-induced protein-like factor ELISA KIT ,Mouse acireductone dioxygenase 1 ELISA KIT
Intended use
Mouse ADI1 ELISA kit allows for the in vitro quantitative determination of Mouse 1,2- dihydroxy- 3- keto- 5- methylthiopentene dioxygenase concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
Mouse ADI1 ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADI1, During the reaction, ADI1 in the sample or standard competes with a fixed amount of biotin-labeled ADI1 for sites on a pre-coated Monoclonal antibody specific to ADI1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ADI1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Delivery time 4-6month
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