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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Protein lin-28 homolog B, Lin28b
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Protein lin- 28 homolog B, LIN28B ELISA Kit
Catalog No:
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
LIN28B,CSDD2; Lin-28.2; lin-28B; protein lin-28 homolog B; lin-28 homolog B (C. elegans)
Search name
Mouse LIN28B ELISA KIT ,Mouse CSDD2 ELISA KIT ,Mouse Lin-28.2 ELISA KIT ,Mouse lin-28B ELISA KIT ,Mouse protein lin-28 homolog B ELISA KIT ,Mouse lin-28 homolog B (C. elegans) ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Protein lin- 28 homolog B, LIN28B beta concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Protein lin- 28 homolog B, LIN28B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Mouse Protein lin- 28 homolog B, LIN28B and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Protein lin- 28 homolog B, LIN28B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse Protein lin- 28 homolog B, LIN28B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
stock solution Nutrient-rich fly medium Phosphate-buffered saline (PBS; pH 7.5) Poly-L-lysine solution (0.1% [w/v] in H2 O; Sigma P8920) Sodium phosphate buffer (10 mM, pH 6.8) Triton X-100 VECTASTAIN Elite ABC Kit
Primer Premier: Program for Design of Degenerate Primers from a Protein Sequence
the Primer Premier primer design program. The surface antigen (HBsAg) protein sequence of the adw strain of hepatitis B virus (HBV) was reverse-translated using the program. The sequence was scanned, and primers were designed in regions of low degeneracy
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