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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
T-cell ecto-ADP-ribosyltransferase 2, Art2b
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse T- cell ecto- ADP- ribosyltransferase 2, Art2b ELISA KIT
Product Name:Mouse T- cell ecto- ADP- ribosyltransferase 2, Art2b ELISA KIT
Packing:96T
Catalog No.:ELI-22329m
Gene Name:Mouse Art2b
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Art2b
Alternative Name:Mouse T-cell ecto-ADP-ribosyltransferase 2, Art2b
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse T- cell ecto- ADP- ribosyltransferase 2, Art2b ELISA KIT allows for the in vitro quantitative determination of Mouse Art2b concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse T- cell ecto- ADP- ribosyltransferase 2, Art2b ELISA KIT has been pre-coated with an Mouse T-cell ecto-ADP-ribosyltransferase 2, Art2b antibody specific to Mouse Art2b .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Art2b and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Art2b , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Art2b in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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. to 1 liter. 4. 30% H2O2 5. OPD Stock, 4.0% 4 g OPD in 100 ml di-H2O. Aliquot and store at -20oC. Protect from light. 6. 4.5N H2SO4 12.0 ml Concentrated Sulfuric Acid 88.0 ml di-H2O 7. Hanks Balanced Salt Solution (HBSS) B. Procedure 1. Wash ELISA
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