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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
60 kDa SS-A
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse 60 kDa SS- A/Ro ribonucleoprotein, Trove2 ELISA KIT
Product Name:Mouse 60 kDa SS- A/Ro ribonucleoprotein, Trove2 ELISA KIT
Packing:96T
Catalog No.:ELI-21633m
Gene Name:Mouse Trove2
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Trove2
Alternative Name:Mouse 60 kDa SS-A/Ro ribonucleoprotein, Trove2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse 60 kDa SS- A/Ro ribonucleoprotein, Trove2 ELISA KIT allows for the in vitro quantitative determination of Mouse Trove2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse 60 kDa SS- A/Ro ribonucleoprotein, Trove2 ELISA KIT has been pre-coated with an Mouse 60 kDa SS-A/Ro ribonucleoprotein, Trove2 antibody specific to Mouse Trove2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Trove2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Trove2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Trove2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验10kDa 干扰素γ诱导蛋白(IP10)在牛生物样本中的精确检测实验
为了便于计算,尽管浓度为自变量而 O.D. 值为因变量,我们绘图时仍采用标准品的 O.D. 值作为横坐标 (X 轴),标准品的浓度为纵坐标 (Y 轴)。同时为了试验结果的直观性,图中提供的是原始数据而非对数值。推荐使用对数 值建立标准曲线图。由于实验操作条件的不同(如操作者、移液技术、洗板技术和温度条件等),标准曲线的 O.D. 值会有所差异。所提供的标准曲线仅供参考,实验者需要根据自已的实验建立标准曲线。牛 10kDa 干扰素γ诱导蛋白 (IP10) 检测试剂盒标准曲线检测
huaxiflame05 各位,我的实验最近遇到了问题,检测一个趋化因子,Western 重复性很差,只有蛋白上样量接近200ug的时候才能勉勉强强看到很弱的条带,并且杂带很多。师兄建议让公司合成纯化蛋白。但问题是原核表达的蛋白分子量35kDa,在真核系统里,这个蛋白质高度糖基化,分子量可达到60kD。在这种情况下,如何构建阳性对照呢? ***解答 magichunter 换成单克隆抗体应该特异性更好
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
) polypeptide A, 220kDa (POLR2A), mRNA 753 NM_001016 Homo sapiens ribosomal protein S12 (RPS12), mRNA 8265 NM_002140 Homo sapiens heterogeneous nuclear ribonucleoprotein K (HNRPK), transcript variant 1, mRNA 2429 NM_002138 Homo sapiens heterogeneous
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