E3 ubiquitin ligase RNF4, Rnf4
ELISA KIT/ Mouse E3 ubiquitin ligase RNF4, Rnf4 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-21628m
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
E3 ubiquitin ligase RNF4, Rnf4
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse E3 ubiquitin ligase RNF4, Rnf4 ELISA KIT
Product Name:Mouse E3 ubiquitin ligase RNF4, Rnf4 ELISA KIT
Packing:96T
Catalog No.:ELI-21628m
Gene Name:Mouse Rnf4
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Rnf4
Alternative Name:Mouse E3 ubiquitin ligase RNF4, Rnf4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse E3 ubiquitin ligase RNF4, Rnf4 ELISA KIT allows for the in vitro quantitative determination of Mouse Rnf4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse E3 ubiquitin ligase RNF4, Rnf4 ELISA KIT has been pre-coated with an Mouse E3 ubiquitin ligase RNF4, Rnf4 antibody specific to Mouse Rnf4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Rnf4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Rnf4 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Rnf4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Reconstitution of CHIP E3 Ubiquitin Ligase Activity
CHIP, the carboxyl-terminus of Hsp70 interacting protein, is both an E3 ubiquitin ligase and an Hsp70 co-chaperone and is implicated in the degradation of cytosolic quality control and numerous disease substrates. CHIP has been shown
Studies of the Ubiquitin Proteasome System
6.3 ) Alternate Protocol 2: Determination of Ubiquitin Chain Variant Phenotype 20× ubiquitylation buffer 2 for E3 assays (see recipe ) Mutant ubiquitins: lysine‐less (K0), K48R, K63R, K48 only, K63 only (BioMol
Functional Assays on High‐Content Protein Microarrays
Figure 2. Schematic of the ubiquitin ligase profiling workflow. A typical assay involves probing five arrays. Three of the arrays are probed with E3 ligases at three different concentrations. The other two arrays are negative controls
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