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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Tetratricopeptide repeat protein 32, Ttc32
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Tetratricopeptide repeat protein 32, Ttc32 ELISA KIT
Product Name:Mouse Tetratricopeptide repeat protein 32, Ttc32 ELISA KIT
Packing:96T
Catalog No.:ELI-16662m
Gene Name:Mouse Ttc32
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Ttc32
Alternative Name:Mouse Tetratricopeptide repeat protein 32, Ttc32
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Tetratricopeptide repeat protein 32, Ttc32 ELISA KIT allows for the in vitro quantitative determination of Mouse Ttc32 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Tetratricopeptide repeat protein 32, Ttc32 ELISA KIT has been pre-coated with an Mouse Tetratricopeptide repeat protein 32, Ttc32 antibody specific to Mouse Ttc32 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ttc32 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ttc32 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ttc32 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
ribosomal protein S5 (RPS5), mRNA 6739 NM_003021 Homo sapiens small glutamine-rich tetratricopeptide repeat (TPR)-containing (SGT), mRNA 488 NM_004285 Homo sapiens hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase) (H6PD), mRNA 646 NM_004142
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
D. Pellet trypsinized cells at 200 x g for 5 min. E. Remove medium and wash cell pellet with HBSS or 1X PBS. F. Centrifuge at 200 x g for 5 min and aspirate wash. G. Repeat wash step of cell
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