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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Poly(A) polymerase gamma, Papolg
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Poly(A) polymerase gamma, Papolg ELISA KIT
Product Name:Mouse Poly(A) polymerase gamma, Papolg ELISA KIT
Packing:96T
Catalog No.:ELI-16197m
Gene Name:Mouse Papolg
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Papolg
Alternative Name:Mouse Poly(A) polymerase gamma, Papolg
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Poly(A) polymerase gamma, Papolg ELISA KIT allows for the in vitro quantitative determination of Mouse Papolg concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Poly(A) polymerase gamma, Papolg ELISA KIT has been pre-coated with an Mouse Poly(A) polymerase gamma, Papolg antibody specific to Mouse Papolg .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Papolg and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Papolg , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Papolg in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Use of the Sleeping Beauty Transposon System for Stable Gene Expression in Mouse Embryonic Stem Cells Ann E. Davidson1 , Theresa E. Gratsch2 , Maria H. Morell2 , K. Sue O’Shea2 , and Catherine E. Krull1 ,3 1 Department
Avoiding DNA Contamination in RT-PCR
), a one-step extraction (Tri Reagent), a filter-binding based extraction (RNAqueous™), by centrifugation through a CsCl cushion, and by two rounds of oligo d(T) selection using Ambion's Poly(A)Pure™ Kit (see Figure 1a ). Regardless of whether reverse
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
acyltransferase, alpha) (AGPAT1), mRNA 742 NM_021134 Homo sapiens mitochondrial ribosomal protein L23 (MRPL23), mRNA 771 NM_021974 Homo sapiens polymerase (RNA) II (DNA directed) polypeptide F (POLR2F), mRNA 1169 NM_006808 Homo sapiens protein
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