- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-16169m
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
D-amino-acid oxidase, Dao
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse D- amino- acid oxidase, Dao ELISA KIT
Product Name:Mouse D- amino- acid oxidase, Dao ELISA KIT
Packing:96T
Catalog No.:ELI-16169m
Gene Name:Mouse Dao
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Dao
Alternative Name:Mouse D-amino-acid oxidase, Dao
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse D- amino- acid oxidase, Dao ELISA KIT allows for the in vitro quantitative determination of Mouse Dao concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse D- amino- acid oxidase, Dao ELISA KIT has been pre-coated with an Mouse D-amino-acid oxidase, Dao antibody specific to Mouse Dao .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Dao and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Dao , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Dao in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Methods for the Detection of D-Amino-Acid Oxidase
that the mouse does not have this enzyme in its liver (13 ). The mouse is a very unique animal in this respect. Northern hybridization, RT-PCR, and western blotting gave positive results for the presence of D-amino-acid oxidase in the ddY/DAO- mice (12 , 17
Methods for the Detection of D-Amino-Acid Oxidase
( 2 , 3 ). In previous work, my colleagues and I concluded that D-amino-acid oxidase is not present in the mouse liver ( 13 ). This paper examines in greater detail the methods used in reaching that conclusion. 4 ). Organs, tissues, or tissue culture cells
). DAOC production level is almost equivalent to the total β-lactams biosynthesized by the parental strain. DAOC deacylation is carried out by two final enzymatic bioconversions catalyzed by D-amino acid oxidase (DAO) and glutaryl acylase (GLA), yielding
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