PHD finger protein 11-like, Ph
f11l ELISA KIT/ Mouse PHD finger protein 11-like, Phf11l ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-16011m
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
PHD finger protein 11-like, Phf11l
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse PHD finger protein 11- like, Phf11l ELISA KIT
Product Name:Mouse PHD finger protein 11- like, Phf11l ELISA KIT
Packing:96T
Catalog No.:ELI-16011m
Gene Name:Mouse Phf11l
Detect Range:15.6-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Phf11l
Alternative Name:Mouse PHD finger protein 11-like, Phf11l
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse PHD finger protein 11- like, Phf11l ELISA KIT allows for the in vitro quantitative determination of Mouse Phf11l concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse PHD finger protein 11- like, Phf11l ELISA KIT has been pre-coated with an Mouse PHD finger protein 11-like, Phf11l antibody specific to Mouse Phf11l .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Phf11l and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Phf11l , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Phf11l in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Gene Editing in One‐Cell Embryos by Zinc‐Finger and TAL Nucleases
., and Cui, X. 2010. Targeted genome modification in mice using zinc‐finger nucleases. Genetics 186:451‐459. Cermak, T., Doyle, E.L., Christian, M., Wang, L., Zhang, Y., Schmidt, C
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
stock solution Nutrient-rich fly medium Phosphate-buffered saline (PBS; pH 7.5) Poly-L-lysine solution (0.1% [w/v] in H2 O; Sigma P8920) Sodium phosphate buffer (10 mM, pH 6.8) Triton X-100 VECTASTAIN Elite ABC Kit
DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
for 30 seconds. 11. Centrifuge at maximum speed ($13,000 x g) at 4°C for 3 minutes. Aspirate the supernatant and air dry the pellet by inverting the tube on a absorbent paper for 5-10 minutes. 12. Add 50-100 μl of nuclease free water
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