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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Mitochondrial ribonuclease P protein 3, Kiaa0391
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Mitochondrial ribonuclease P protein 3, Kiaa0391 ELISA KIT
Product Name:Mouse Mitochondrial ribonuclease P protein 3, Kiaa0391 ELISA KIT
Packing:96T
Catalog No.:ELI-15915m
Gene Name:Mouse Kiaa0391
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Kiaa0391
Alternative Name:Mouse Mitochondrial ribonuclease P protein 3, Kiaa0391
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Mitochondrial ribonuclease P protein 3, Kiaa0391 ELISA KIT allows for the in vitro quantitative determination of Mouse Kiaa0391 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Mitochondrial ribonuclease P protein 3, Kiaa0391 ELISA KIT has been pre-coated with an Mouse Mitochondrial ribonuclease P protein 3, Kiaa0391 antibody specific to Mouse Kiaa0391 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Kiaa0391 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Kiaa0391 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Kiaa0391 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
5-monooxygenase activation protein, eta polypeptide (YWHAH), mRNA 2195 NM_002939 Homo sapiens ribonuclease/angiogenin inhibitor (RNH), mRNA 726 NM_003562 Homo sapiens solute carrier family 25 (mitochondrial carrier; oxoglutarate carrier), member 11 (SLC
isolation kit, Qiagen), pooled mRNA for each treatment group. As well as GAPDH, factor VII, glucose-6-phosphatase and VEGF mRNAs were also used for normalization. ELISA was used to quantify the reduction of apoB-100 protein levels in mouse plasma
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