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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Malignant T cell-amplified sequence 1, Mcts1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Malignant T cell- amplified sequence 1, MCTS1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Malignant T cell-amplified sequence 1(MCT-1),Multiple copies T-cell malignancies, Mcts1,MCT 1,MCT-1; MCT1; malignant T-cell-amplified sequence 1; multiple copies T-cell malignancies; malignant T cell amplified sequence 1
Search name
Mouse Malignant T cell-amplified sequence 1(MCT-1) ELISA KIT ,Mouse Multiple copies T-cell malignancies ELISA KIT ,Mouse Mcts1 ELISA KIT ,Mouse MCT 1 ELISA KIT ,Mouse MCT-1 ELISA KIT ,Mouse MCT1 ELISA KIT ,Mouse malignant T-cell-amplified sequence 1 ELISA KIT ,Mouse multiple copies T-cell malignancies ELISA KIT ,Mouse malignant T cell amplified sequence 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse malignant t cell-amplified sequence 1,MCT-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to MCT-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MCT-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MCT-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MCT-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Using Bioinformatics Tools for the Sequence Analysis of Immunoglobulins and T Cell Receptors
Blake, J.A., Richardson, J.E., Bult, C.J., Kadin, J.A., Eppig, J.T., and Mouse Genome Database Group. 2003. MGD: The Mouse Genome Database. Nucl. Acids Res. 31:193‐195.
During T-cell maturation, T-cell receptor (TCR) gene segments rearrange, resulting in a new, unique DNA configuration. The recombined TCR gene loci display a high degree of nucleotide sequence variability. Molecular biological clonality assays
Genetic Vaccination Targeting T-Cell Receptors
T-cell antigen receptor (TCR) genes (which consist of variable (V), diversity (D), joining (J) and constant (C) segments) undergo rearrangement during T-cell development and result in the expression of a disulfide linked heterodimer (α and �
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