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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Proteasome subunit beta type-7, Psmb7
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Proteasome subunit beta type- 7, Psmb7 ELISA KIT
Product Name:Mouse Proteasome subunit beta type- 7, Psmb7 ELISA KIT
Packing:96T
Catalog No.:ELI-15650m
Gene Name:Mouse Psmb7
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Psmb7
Alternative Name:Mouse Proteasome subunit beta type-7, Psmb7
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Proteasome subunit beta type- 7, Psmb7 ELISA KIT allows for the in vitro quantitative determination of Mouse Psmb7 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Proteasome subunit beta type- 7, Psmb7 ELISA KIT has been pre-coated with an Mouse Proteasome subunit beta type-7, Psmb7 antibody specific to Mouse Psmb7 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Psmb7 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Psmb7 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Psmb7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Studies of the Ubiquitin Proteasome System
or other proteasome inhibitor (e.g., lactacystin; Calbiochem or Biomol) Phosphate‐buffered saline (PBS; see recipe ) Nondenaturing Triton X‐100 lysis buffer (see recipe ) containing 1×
Genetic and Pharmacologic Models for Type 1 Diabetes
. Mouse models of diabetic nephropathy. J. Am. Soc. Nephrol. 20: 2503‐ 2512. Bugger, H. , Chen, D. , Riehle, C. , Soto, J. , Theobald, H.A. , Hu, X.X. , Ganesan, B. , Weimer, B
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
) subunit, beta type, 7 (PSMB7), mRNA 545 NM_002300 Homo sapiens lactate dehydrogenase B (LDHB ), mRNA 4144 NM_004176 Homo sapiens sterol regulatory element binding transcription factor 1 (SREBF1), mRNA 632 NM_002796 Homo sapiens proteasome (prosome
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