Sodium channel subunit beta-1,
Scn1b ELISA KIT/ Mouse Sodium channel subunit beta-1, Scn1b ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-15470m
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Sodium channel subunit beta-1, Scn1b
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Sodium channel subunit beta- 1, Scn1b ELISA KIT
Product Name:Mouse Sodium channel subunit beta- 1, Scn1b ELISA KIT
Packing:96T
Catalog No.:ELI-15470m
Gene Name:Mouse Scn1b
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Scn1b
Alternative Name:Mouse Sodium channel subunit beta-1, Scn1b
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Sodium channel subunit beta- 1, Scn1b ELISA KIT allows for the in vitro quantitative determination of Mouse Scn1b concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Sodium channel subunit beta- 1, Scn1b ELISA KIT has been pre-coated with an Mouse Sodium channel subunit beta-1, Scn1b antibody specific to Mouse Scn1b .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Scn1b and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Scn1b , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Scn1b in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Phycoerythrin conjugation protocol
fluorescence intensity in the FL2 (orange fluorescence) channel is shown. References Chambers JD, Simon SI, Berger EM, Sklar LA, Arfors K-E. Endocytosis of beta2 integrins by stimulated human neutrophils analyzed by flow cytometry. Journal
, the discs were washed for 10 minutes each with the following buffers: 50% formamide, 2xSSC (30mM sodium citrate dihydrate, 0.3M sodium chlorine), 0.1% Triton X-100, 0.3% CHAPS (3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate) (wash buffer
Studies of the Ubiquitin Proteasome System
and Purification of Anti‐Ubiquitin Antibodies Support Protocol 3: Preparation of Ubiquitin Affinity Column Generation of Reagent‐Grade Ubiquitin Activating Enzyme (E1) Basic Protocol 20: Preparation of Wheat E1 in E. coli Basic Protocol 21: Preparation of Mouse E1
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