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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Selenoprotein K, Selk
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Selenoprotein K, Selk ELISA KIT
Product Name:Mouse Selenoprotein K, Selk ELISA KIT
Packing:96T
Catalog No.:ELI-15466m
Gene Name:Mouse Selk
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Selk
Alternative Name:Mouse Selenoprotein K, Selk
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Selenoprotein K, Selk ELISA KIT allows for the in vitro quantitative determination of Mouse Selk concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Selenoprotein K, Selk ELISA KIT has been pre-coated with an Mouse Selenoprotein K, Selk antibody specific to Mouse Selk .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Selk and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Selk , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Selk in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂,增加了操作步骤,在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法:1, TORCH及传染病试剂盒(间接法),见2.2.32, TORCH-IgM捕获法特色:包被抗体,标记抗原原理:3, 细胞因子试剂盒采用的方法路线(ABC-ELISA)原理产品特色:采用ABC法,灵敏度更高,特异性更强。生物素抗体和酶联物是浓缩的,使用前需用相应的缓冲液稀释。酶联物可以通用
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
Performing Bronchoalveolar Lavage in the Mouse
and inflammatory cell populations; it is indispensable for studying cell influx in disease models of the airways such as asthma and COPD. Cell counts can be combined with methods such as ELISA, immunoblot, immunohistochemistry, quantitative polymerase chain
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