Peptidyl-prolyl cis-trans isom
erase C, Ppic ELISA KIT/ Mouse Peptidyl-prolyl cis-trans isomerase C, Ppic ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-15320m
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Peptidyl-prolyl cis-trans isomerase C, Ppic
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Peptidyl- prolyl cis- trans isomerase C, Ppic ELISA KIT
Product Name:Mouse Peptidyl- prolyl cis- trans isomerase C, Ppic ELISA KIT
Packing:96T
Catalog No.:ELI-15320m
Gene Name:Mouse Ppic
Detect Range:15.62-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Ppic
Alternative Name:Mouse Peptidyl-prolyl cis-trans isomerase C, Ppic
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Peptidyl- prolyl cis- trans isomerase C, Ppic ELISA KIT allows for the in vitro quantitative determination of Mouse Ppic concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Peptidyl- prolyl cis- trans isomerase C, Ppic ELISA KIT has been pre-coated with an Mouse Peptidyl-prolyl cis-trans isomerase C, Ppic antibody specific to Mouse Ppic .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ppic and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ppic , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ppic in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验伴侣有： GroES-GroEL DnaK-DnaJ-GrpE ClpB 常用的折叠酶有： peptidyl prolyl cis/trans isomerases (PPI's) disulfide oxidoreductase (DsbA) and disulfide isomerase (DsbC) protein disulfide isomerase
prolyl cis-trans isomerase，PPI)。以PDI为例，众所周知，蛋白质分子中的二硫键与新生肽段的折叠密切相关，对维系蛋白质分子的结构稳定性和功能发挥也有重要作用。PDI定位在内质网管腔内，含量丰富，催化蛋白质分子内巯基与二硫键之间的交换反应。同时，它是目前发现的最为突出的多功能蛋白，除了二硫键的异构酶的基本功能外，它还是脯氨酸-4-羟化酶的α亚基;又是微粒体内甘油三酯转移蛋白复合物的小亚基，还是一种糖基化位点结合蛋白(gkycisylation site binding
异构酶(peptidyl prolyl cis-trans isomerase，PPI)。以PDI为例，众所周知，蛋白质分子中的二硫键与新生肽段的折叠密切相关，对维系蛋白质分子的结构稳定性和功能发挥也有重要作用。PDI定位在内质网管腔内，含量丰富，催化蛋白质分子内巯基与二硫键之间的交换反应。同时，它是目前发现的最为突出的多功能蛋白，除了二硫键的异构酶的基本功能外，它还是脯氨酸-4-羟化酶的α亚基;又是微粒体内甘油三酯转移蛋白复合物的小亚基，还是一种糖基化位点结合蛋白(gkycisylation site
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