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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Ras-like protein family member 10B, Rasl10b
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Ras- like protein family member 10B, Rasl10b ELISA KIT
Product Name:Mouse Ras- like protein family member 10B, Rasl10b ELISA KIT
Packing:96T
Catalog No.:ELI-15212m
Gene Name:Mouse Rasl10b
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Rasl10b
Alternative Name:Mouse Ras-like protein family member 10B, Rasl10b
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Ras- like protein family member 10B, Rasl10b ELISA KIT allows for the in vitro quantitative determination of Mouse Rasl10b concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Ras- like protein family member 10B, Rasl10b ELISA KIT has been pre-coated with an Mouse Ras-like protein family member 10B, Rasl10b antibody specific to Mouse Rasl10b .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Rasl10b and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Rasl10b , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Rasl10b in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
Homo sapiens RAP1B, member of RAS oncogene family (RAP1B), mRNA 736 NM_016532 Homo sapiens skeletal muscle and kidney enriched inositol phosphatase (SKIP), transcript variant 1, mRNA 1529 NM_015292 Homo sapiens likely ortholog of mouse membrane
secondary antibody review -- data from 99 publications
epithelial Caco2/bbe cells Bio-Rad 10 rhodamine immunocytochemistry 1:100 detect phospho-CREB in human colonic epithelial Caco2/bbe cells Bio-Rad 11 goat IgG FITC immunohistochemistry 1:40 detect ameloblastin in mouse postnatal mandibular molars
祝贺〖雅裕睿安〗成为Signalway Antibody(SAB)独家
Xia''Xiang Gao''Zhimin Lu''et al.(2009) FAK Phosphorylation by ERK Primes Ras-Induced Tyrosine Dephosphorylation of FAK Mediated by PIN1 and PTP-PEST.Molecular Cell''35:11–25 3.J Bossuyt''K Helmstadter''X Wu''et al.(2008) u Ca
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