Osteoclast-stimulating factor
1, Ostf1 ELISA KIT/ Mouse Osteoclast-stimulating factor 1, Ostf1 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-14801m
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Osteoclast-stimulating factor 1, Ostf1
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Osteoclast- stimulating factor 1, Ostf1 ELISA KIT
Product Name:Mouse Osteoclast- stimulating factor 1, Ostf1 ELISA KIT
Packing:96T
Catalog No.:ELI-14801m
Gene Name:Mouse Ostf1
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Ostf1
Alternative Name:Mouse Osteoclast-stimulating factor 1, Ostf1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Osteoclast- stimulating factor 1, Ostf1 ELISA KIT allows for the in vitro quantitative determination of Mouse Ostf1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Osteoclast- stimulating factor 1, Ostf1 ELISA KIT has been pre-coated with an Mouse Osteoclast-stimulating factor 1, Ostf1 antibody specific to Mouse Ostf1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ostf1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ostf1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ostf1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Osteoclast Formation in the Mouse Coculture Assay
) to stimulate RANKL and macrophage colony-stimulating factor (M-CSF) expression. These factors then stimulate early osteoclast precursors present in the spleen or bone marrow cell populations to differentiate into mature osteoclasts. At the end of the culture
Generation of Osteoclasts In Vitro, and Assay of Osteoclast Activity
: receptor activator of nuclear factor κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF). Using RANKL and M-CSF, we can induce osteoclasts from monocytemacrophage lineage cells even in the absence of osteoblasts. This chapter describes
Generating Murine Osteoclasts from Bone Marrow
-stimulating factor (M-CSF, also called CSF-1) produced by osteoblasts/stromal cells was shown to be an essential factor for differentiation of osteoclasts from osteoclast progenitors (2 ,5 ). Recently, receptor activator of nuclear factor κB ligand (RANKL
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