- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-14757m
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Zinc finger protein 2, Zfp2
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Zinc finger protein 2, Zfp2 ELISA KIT
Product Name:Mouse Zinc finger protein 2, Zfp2 ELISA KIT
Packing:96T
Catalog No.:ELI-14757m
Gene Name:Mouse Zfp2
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Zfp2
Alternative Name:Mouse Zinc finger protein 2, Zfp2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Zinc finger protein 2, Zfp2 ELISA KIT allows for the in vitro quantitative determination of Mouse Zfp2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Zinc finger protein 2, Zfp2 ELISA KIT has been pre-coated with an Mouse Zinc finger protein 2, Zfp2 antibody specific to Mouse Zfp2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Zfp2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Zfp2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Zfp2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验specificity of each fusion protein was tested by ELISA using a panel of DNA substrates (Fig. 2B,C). The predicted DNA binding site of each TFZF was decoded from the -helical sequence of the corresponding zinc finger (Table 1). As expected, the majority
. For example, novel DNA-binding proteins have been created by using phage display to alter the DNA-binding specificity of a zinc-finger protein (ZFP)2-7 . Although phage display is an effective method, it is labor-intensive and time-consuming
. PCR cycling conditions in a PE 9600 thermocycler (see TIP 2) TIP 1: In the published work cited above, degenerate primers directed against zinc finger motifs were used in conjunction with Stratagene's RAP-PCR arbitrary primers. To generalize
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