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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Paired box protein Pax-4, Pax4
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Paired box protein Pax- 4, Pax4 ELISA KIT
Product Name:Mouse Paired box protein Pax- 4, Pax4 ELISA KIT
Packing:96T
Catalog No.:ELI-14716m
Gene Name:Mouse Pax4
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Pax4
Alternative Name:Mouse Paired box protein Pax-4, Pax4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Paired box protein Pax- 4, Pax4 ELISA KIT allows for the in vitro quantitative determination of Mouse Pax4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Paired box protein Pax- 4, Pax4 ELISA KIT has been pre-coated with an Mouse Paired box protein Pax-4, Pax4 antibody specific to Mouse Pax4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Pax4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Pax4 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Pax4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Differentiation and Lineage Commitment of Murine Embryonic Stem Cells into Insulin Producing Cells
ectopically expressing pancreatic and duodenal homeobox 1 (Pdx1) and paired box gene 4 (Pax4).
Rational Design and Evaluation of FRET Experiments to Measure Protein Proximities in Cells
). The histograms to the right represent relative FRET efficiencies for each situation. Equal numbers of paired squares and circles (A) yields equivalent FRET values regardless of which protein is the donor or acceptor. In contrast, a substoichiometric number
Demidov, V.V., Dokholyan, N.V., Witte‐Hoffmann, C., Chalasani, P., Yiu, H.W., Ding, F., Yu, Y., Cantor, C.R., and Broude, N.E. 2006. Fast complementation of split fluorescent protein triggered by DNA hybridization. Proc. Natl. Acad. Sci. U.S
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