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- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Serine
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Serine/threonine- protein kinase PAK 3, Pak3 ELISA KIT
Product Name:Mouse Serine/threonine- protein kinase PAK 3, Pak3 ELISA KIT
Packing:96T
Catalog No.:ELI-14684m
Gene Name:Mouse Pak3
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Pak3
Alternative Name:Mouse Serine/threonine-protein kinase PAK 3, Pak3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Serine/threonine- protein kinase PAK 3, Pak3 ELISA KIT allows for the in vitro quantitative determination of Mouse Pak3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Serine/threonine- protein kinase PAK 3, Pak3 ELISA KIT has been pre-coated with an Mouse Serine/threonine-protein kinase PAK 3, Pak3 antibody specific to Mouse Pak3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Pak3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Pak3 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Pak3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验-dependent kinase inhibitor 1A 4. 在大鼠芯片 list 中查找 Cdkn1a,得 :13 17 n0313f03 U33314 Rn.10128 Rattus norvegicus serine/threonine kinase beta-PAK mRNA, complete cds p21 (CDKN1A)-activated kinase 3 Pak3 就这样找到了和人的 p21 基因对应的大鼠基因 Cdkn1a. 如果你对该序列
-89 个氨基酸的N末端调节区的部分p21-activated kinase (PAK-1)相结合。PAK-1的这个区域,被认为是p21偶联区域即P21binding domain (PBD),特异性的与活性形式的Rac1相作用,最终提供了一个优秀的工具以分离细胞样本中的活性Rac1. 细胞被镁盐缓冲溶液( magnesium lysis buffer)所裂解; 接着裂解液需要采用GST-agarose beads (not provided)进行预清除.在这一步,少量的细胞裂解液能够储存
CUSABIO季度文献盘点:二季度新增600篇产品引用文献,总文献数量已达 31400篇!
标题:Severe fever with thrombocytopenia syndrome virus infection of the male reproductive tract induces pathology and inflammation 影响因子:19.4 发表期刊:Nature Microbiology CUSABIO助力产品: Mouse serum amyloid A-3 protein (SAA3) ELISA kit; CSB-EL020658MOMouse S100
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