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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
RING finger protein 37, Ubox5
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse RING finger protein 37, Ubox5 ELISA KIT
Product Name:Mouse RING finger protein 37, Ubox5 ELISA KIT
Packing:96T
Catalog No.:ELI-14683m
Gene Name:Mouse Ubox5
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Ubox5
Alternative Name:Mouse RING finger protein 37, Ubox5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse RING finger protein 37, Ubox5 ELISA KIT allows for the in vitro quantitative determination of Mouse Ubox5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse RING finger protein 37, Ubox5 ELISA KIT has been pre-coated with an Mouse RING finger protein 37, Ubox5 antibody specific to Mouse Ubox5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ubox5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ubox5 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ubox5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
stock solution Nutrient-rich fly medium Phosphate-buffered saline (PBS; pH 7.5) Poly-L-lysine solution (0.1% [w/v] in H2 O; Sigma P8920) Sodium phosphate buffer (10 mM, pH 6.8) Triton X-100 VECTASTAIN Elite ABC Kit
Studies of the Ubiquitin Proteasome System
and Translation Systems In Vitro Production of Ubiquitin‐Protein Ligases (E3s) Basic Protocol 24: Expression of Ring‐Finger E3s in Bacterial Lysates Alternate Protocol 14: Expression of HECT and U‐Box E3s in Bacterial Lysates Alternate Protocol 15: Expression
) resource provides the research community with access to information on the genetics, genomics, and biology of the laboratory mouse. Core data in MGI include gene characterization and function, phenotype and disease model descriptions, DNA and protein
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