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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Rho GTPase-activating protein 28, Arhgap28
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Rho GTPase- activating protein 28, Arhgap28 ELISA KIT
Product Name:Mouse Rho GTPase- activating protein 28, Arhgap28 ELISA KIT
Packing:96T
Catalog No.:ELI-14577m
Gene Name:Mouse Arhgap28
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Arhgap28
Alternative Name:Mouse Rho GTPase-activating protein 28, Arhgap28
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Rho GTPase- activating protein 28, Arhgap28 ELISA KIT allows for the in vitro quantitative determination of Mouse Arhgap28 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Rho GTPase- activating protein 28, Arhgap28 ELISA KIT has been pre-coated with an Mouse Rho GTPase-activating protein 28, Arhgap28 antibody specific to Mouse Arhgap28 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Arhgap28 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Arhgap28 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Arhgap28 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Assessment of Rho GTPase Signaling During Neurite Outgrowth
Rho GTPases are key regulators of the cytoskeleton during the process of neurite outgrowth. Based on overexpression of dominant-positive and negative Rho GTPase constructs, the classic view is that Rac1 and Cdc42 are important for neurite
Rho GTPase Knockout Induction in Primary Keratinocytes from Adult Mice
Primary keratinocytes are an important tool to investigate the molecular mechanism underlying the skin phenotype of mice with null mutations in Rho GTPase genes. If the RhoA gene deletion is conditional, the knockout can be induced in vitro
Functional Analysis of Rho GTPase Activation and Inhibition in a Bead-Based Miniaturized Format
Extensive knowledge about protein–protein interactions is fundamental to fully understand signaling pathways and for the development of new drugs. Rho GTPases are key molecules in cellular signaling processes and their deregulation
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