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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Leukotriene B4 receptor 2, Ltb4r2
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Leukotriene B4 receptor 2, LTB4R2 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
LTB4R2,BLT2; BLTR2; JULF2; KPG_004; LTB4-R 2; LTB4-R2; NOP9; LTB4 receptor JULF2; leukotriene B4 receptor BLT2; seven transmembrane receptor BLTR2; leukotriene B4 receptor 2
Search name
Mouse LTB4R2 ELISA KIT ,Mouse BLT2 ELISA KIT ,Mouse BLTR2 ELISA KIT ,Mouse JULF2 ELISA KIT ,Mouse KPG_004 ELISA KIT ,Mouse LTB4-R 2 ELISA KIT ,Mouse LTB4-R2 ELISA KIT ,Mouse NOP9 ELISA KIT ,Mouse LTB4 receptor JULF2 ELISA KIT ,Mouse leukotriene B4 receptor BLT2 ELISA KIT ,Mouse seven transmembrane receptor BLTR2 ELISA KIT ,Mouse leukotriene B4 receptor 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Leukotriene B4 receptor 2, LTB4R2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leukotriene B4 receptor 2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Leukotriene B4 receptor 2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Leukotriene B4 receptor 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Leukotriene B4 receptor 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized Product
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文献和实验Performing Bronchoalveolar Lavage in the Mouse
. Activation of the lung S1P1 receptor reduces allergen‐induced plasma leakage in mouse. Brit. J. Pharmacol. 158:1295‐1301. Delayre‐Orthez, C., Becker, J., De Blay, F., Frossard, N
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
Assay for Chemoattractant Binding
) migrate to a variety of chemoattractants, including N-formyl peptides, complement-derived C5a, leukotriene B4 , interleukin-8, and platelet-activating factor, each of which has a distinct receptor on the leukocyte plasma membrane.
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